PEDRO NASCIAMENTO
#212-2431 Avenue Road | Toronto, ON | M5S 2T2
QUALIFICATIONS SUMMARY
• Experienced lab technician with expertise in animal surgical procedures
• Post-graduate diploma in animal science and Registered Laboratory Animal Technician (RLAT) from the Canadian
Council on Animal Care
• Proficient in molecular and microbiology techniques: gel electrophoresis, DNA and RNA extraction, agarose gel
electrophoresis and protein expression and purification
• In-depth knowledge of bio-safety regulations and experienced in training students and researchers on workplace
safety
• Published articles in scientific journals demonstrate excellent written communication skills. Meticulous in
maintaining accurate records in lab journals
LABORATORY COMPETENCIES
PROFESSIONAL EXPERIENCE
Laboratory Technologist II (Animal Lab/Surgical Technician) 2010 – Present
University of Toronto, Faculty of Medicine
• Conduct and assist in brain microsurgical experiments that demonstrate well-developed surgical skills, such as
stereotaxic brain injection/lesioning and cannulation- perfusion
• Developed new anaesthesia technique that led to a change in protocol for surgical procedure for Department of
Animal Laboratories
• Ensure laboratory is safe for students and researchers by applying and renewing bio-safety hazard level 2
standards according to the Ministry of Health
• Proficient in molecular techniques including gel electrophoresis (SDS-PAGE and western blot), DNA and RNA
extraction, protein expression and purification, agarose gel electrophoresis, PCR (conventional, RT-PCR and Real-
time), genotyping, immunofluorescence and confocal microscopy, animal colony management, husbandry and
animal handling (ear tagging and ear notching, weaning).
• Train 4th year students on mastering genotyping to map DNA and RNA genotypes in order to meet the
requirements of laboratory curriculum
• Maintain animal colony and conduct laboratory molecular biology experiments ranging from genotyping to
proteomics
Resume Templates 1210 Document(s)
